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KMID : 0381120200420070773
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Genes and Genomics
2020 Volume.42 No. 7 p.773 ~ p.777
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Genome based quantification of VHSV in multiple organs of infected olive flounder (Paralichthys olivaceus) using real-time PCR
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Kim Hyun-Su
Baek Kyung-Wan
Ko Eun-Ji
Luan Nguyen Thanh
Lim Yun-Jin
Roh Heyong-Jin
Kim Nam-Eun
Kim Do-Hyung
Kim Ah-Ran
Choi Yung-Hyun
Kim Suhk-Mann
Kim Heui-Soo
Ock Mee-Sun
Cha Hee-Jae
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Abstract
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Background: Viral hemorrhagic septicemia (VHS) is a serious viral disease that infects the olive flounder in South Korea. The Korean aquaculture industry experienced an economic loss caused by the high infectivity and mortality.
Objective: This study aimed to evaluate the infection density of VHSV in various organs of the olive flounder including spleen, liver, kidney, stomach, esophagus, intestine, gill, muscle, heart, and brain. Olive flounders were collected from a local fish farm and injected subcutaneously with 106 PFU/fish.
Methods: Each 15 fish were sampled at 0, 3, and 7 days post challenge (dpc), respectively, to perform quantitative analysis of VHSV using SYBR-green based real-time PCR in various tissues including spleen, liver, head-kidney, body-kidney, muscle, esophagus, stomach, intestine, gill, and brain.
Results: Organs infected with VHSV were obtained after 3 and 7 days. Each organs were examined for viral infection using real-time PCR. The data obtained from this experiment revealed copy numbers higher than 10 copies per 100 ng cDNA in the spleen (15.26?±?3.11 copies/100 ng of cDNA), muscle (11.24?±?2.25 copies), and gill (14.23?±?6.26 copies), but lower in liver, head-kidney, body-kidney, esophagus, brain and stomach.
Conclusion: The present study, together with previous data, demonstrated that the gill, spleen, and muscle are the major target organs of VHSV in olive flounder. Therefore, central monitoring of spleen, gill and muscle should be considered and might be necessary if anti-VHSV treatment is to be successful in infected olive flounder.
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KEYWORD
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VHSV, Copy number, Olive flounder, Target organ, Real-time PCR
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